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Analysis of bone ash 2015 Submitted by- Srishti Chauhan Class xii b Submitted to- Mrs. Kiran Pareek
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Acknowledgement I sincerely thank my chem teacher Mrs. Kiran Pareek, for the help and guidance provided to me in completing the project in chemistry. I also thank the principal for permitting the use of the resources of the school for completing the project.
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Certificate Name of princi Name of grade teacher
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Aim S.No. 1 2 3 4 5 6 7 8 9 Analysis of bone ash Index
Requirements Rib Bone 2 Pieces Beaker 150 ml Test Tube 7 nos Evaporating Dish 1 no Ring Stand 1 no Bunsen Burner 1 no Test Tube Holder 2 nos Filter Paper PH Paper Dil. Nitric Acid 200 ml 1% Ammonium Hydroxide 100 ml 1% Silver Nitrate 25 ml 1% Ammonium Chloride 50 ml Acetic Acid 100 ml 1% Potassium Thiocyanate 25 ml Distilled Water As Reqd
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Theme (significance of pro) Deals with analysis of bone ash Provides all basic ideas about concentration of various salts present Provides information about ion exchange reaction
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chem. Eqn
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Procedure
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Observation table experiment A strip of bone was burnt in evaporating dish 2 gms of bone ash was weighed To it dilute nitric acid was added It was diluted with water The above solution was filtered and the residue (left on the filter paper) was discarded Ammonium hydroxide was added to the filtrate (left on the beaker) The solution was made basic. The basicity was checked with the help of pH paper The solution was filtered and the residue was isolated The filtrate was separated into two test tubes Silver nitrate was added to one of the test tubes To the other test tube ammonium chloride and ammonium carbonate was added simultaneously and boiled To the solution left, dilute HCL was added followed by Potassium thiocyanate
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observation Yellowish white precipitate was obtained the ash sparingly dissolved
the ash was completely dissolved
The pH was made to 8.6 Whitish brown precipitate of Magnesium ammonium phosphate was obtained
White precipitate of Silver chloride was obtained White residue of calcium Carbonate was obtained
Red colour solution marking the presence of Iron was obtained
Conclusion Extrapolation from the above observations Constituents of bone ash identified were: I. Calcium II. Phosphate III. Chloride IV. Magnesium V. Iron Apart from this Calcium and Phosphate which is found maximum in bone was estimated from the precipitate got. This was done by weighing the precipitate Weight of Calcium carbonate:- 1.7 g Weight of Mg (NH4) PO4 :- 1.1 g Weight of Ca in 2g of sample:- 0.68 g Weight of Phosphorous:- 0.24 g % of Ca:- 34% % of Phosphorous:- 12%
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Precaution
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Bibliography Ncert chem book www.icbse.com Wikipedia